Discovery of antibodies against difficult targets requires a “more shots on goal” approach in order to yield lead molecules with a desired functional profile. However, traditional hybridoma methods are slow and often do not yield the necessary volume of diversity that is required when tackling difficult targets.

In this application note we demonstrate that the Opto™ Plasma B Discovery workflow can be used to:

• Discover up to 10-fold more hits against soluble and membrane antigens in days not weeks
• Generate diverse lead panels by sampling >50-fold greater sequence diversity
• Increase campaign yield and efficiency to reduce costs by 20-fold